Inside Molecular Cloning: How We Duplicate DNA for Research and Innovation
When most people hear the word cloning, they think of science fiction stories about cloned animals or even humans. But in molecular biology, cloning has a much more precise and practical meaning: it’s about copying specific pieces of DNA so that scientists can study, modify, and use them for research, medicine, and biotechnology.
For decades, molecular cloning has been the foundation of modern biology. It allows scientists to understand genes, make proteins, engineer crops, produce medicines like insulin, and even edit genomes using tools like CRISPR.
In simple terms, molecular cloning is about isolating a piece of DNA like a gene and making many identical copies of it.
This is different from cloning an entire animal (like Dolly the sheep). In molecular biology, you’re only working with the DNA itself, not the whole organism.

Restriction Enzymes
Restriction enzymes also called restriction endonucleases are special proteins that act like precise molecular scissors. They recognize specific DNA sequences and cut the DNA at or near those sites.
These enzymes were first discovered in bacteria, which use them as a defense mechanism to cut up invading viral DNA. The name “restriction” comes from the fact that they restrict or limit the growth of viruses in bacteria.
How Do Restriction Enzymes Work?
Each restriction enzyme identifies a short, specific DNA sequence called a recognition site, which is usually 4–8 base pairs long.
- When the enzyme finds its recognition site on a DNA molecule, it binds to the DNA and makes a precise cut.
- This cut can result in either blunt ends (straight cuts through both strands at the same position) or sticky ends (staggered cuts that leave short, single-stranded overhangs).
DNA ligase
DNA ligase is an enzyme that acts like molecular glue. Its main job is to join (ligate) two pieces of DNA together by sealing breaks in the sugar-phosphate backbone of the DNA strands.
In molecular cloning, DNA ligase makes it possible to link a DNA fragment (like a gene) with a cloning vector (such as a plasmid). Without this step, the inserted DNA wouldn’t stay attached and wouldn’t get copied inside the host cell.
How Does DNA Ligase Work?
DNA ligase catalyzes the formation of a phosphodiester bond, which connects the 3’-hydroxyl end of one nucleotide to the 5’-phosphate end of another.
In practical cloning:
DNA is first cut with restriction enzymes, creating either “sticky ends” (with overhangs) or “blunt ends” (straight cuts).
When the DNA fragment and plasmid are mixed together, their ends base-pair if they match.
DNA ligase seals the nicks in the backbone, making the DNA insert and plasmid one continuous, stable double-stranded circle.

Plasmid Vectors
A plasmid vector is a small, circular piece of double-stranded DNA that is separate from the chromosomal DNA of a cell. Naturally, plasmids are found in bacteria and sometimes in yeast — they often carry extra genes that help bacteria survive, like antibiotic resistance genes.
In molecular cloning, scientists use plasmids as vectors, which means they act as carriers or delivery vehicles to transfer a piece of foreign DNA (like a gene of interest) into a host cell where it can be copied or expressed.
- It picks up the DNA fragment you want to clone.
- It carries it into a host cell (usually E. coli bacteria).
- Once inside, the plasmid uses the host’s cellular machinery to replicate itself — and therefore, the inserted DNA — as the cell grows and divides.
Key Features of a Good Plasmid Vector
Origin of Replication (ori)
This is the DNA sequence that tells the host cell’s machinery where to start copying the plasmid.
Without it, the plasmid won’t replicate.
Selectable Marker
Usually an antibiotic resistance gene (e.g., ampicillin resistance).
It lets scientists grow only the cells that have taken up the plasmid — cells without the plasmid will die when exposed to the antibiotic.
Multiple Cloning Site (MCS)
A short DNA region containing multiple unique restriction enzyme sites.
Allows easy insertion of foreign DNA fragments.
Reporter Gene (Optional)
Sometimes included to help identify whether the DNA fragment was successfully inserted.
Example: LacZ gene (blue-white screening) — colonies with inserts stay white, while empty vectors turn blue.
Small Size
Smaller plasmids are easier for cells to take up and replicate more efficiently.
Common Types of Plasmid Vectors
Cloning Vectors
Designed mainly for inserting and replicating foreign DNA.
Example: pUC19, pBluescript.
Viral Vectors
Based on viruses but modified to be safe.
Used for cloning or gene therapy in more complex cells like mammalian cells.
Expression Vectors
Have extra elements like promoters and ribosome binding sites so the inserted gene is transcribed and translated into protein.
Used when the goal is to produce large amounts of a protein.
Example: pET series, pGEX.
Shuttle Vectors
Can replicate in multiple types of host cells (e.g., bacteria AND yeast).
Useful for moving DNA between species.
What Do Host Cells Do?
In molecular cloning, host cells have three main jobs:
Accept the Recombinant DNA: Host cells must be able to take up the plasmid vector that carries the DNA fragment of interest. This process is called transformation (in bacteria) or transfection (in eukaryotic cells).
Replicate the Recombinant DNA: Once inside, the host cell’s machinery replicates the plasmid along with its own DNA during cell division. This creates a population of cells that all carry identical copies of the cloned gene.
Express the Gene (Optional): Some host cells are also used to express (make) the protein coded by the inserted gene. This is critical for producing things like insulin, enzymes, or antibodies.
Types of Host Cells Used in Cloning

Bacterial Host Cells
Example: Escherichia coli (E. coli)
Rapid growth rate they divide every 20 minutes under ideal conditions.
Easy to transform with plasmids.
Well-understood genetics.
Cheap to grow in simple nutrient media.

Yeast Host Cells
Example: Saccharomyces cerevisiae (Baker’s yeast)
Eukaryotic cells: more similar to plants and animals.
Can perform some post-translational modifications.
Still relatively easy to grow.

Mammalian Host Cells
Example: CHO (Chinese Hamster Ovary) cells, HEK293 (Human Embryonic Kidney) cells.
Most accurate expression of complex human proteins.
Capable of full post-translational modifications (glycosylation, folding).

Other Hosts (Insect & Plant Cells)
Insect Cells: e.g., Sf9 cells with baculovirus expression systems useful for high-yield production of certain proteins.
Plant Cells: Agrobacterium-mediated transformation in plants used to create genetically modified crops.
How PCR Helps in Molecular Cloning
PCR is used at multiple steps in molecular cloning:
Amplify the Target Gene
If you only have a tiny amount of DNA, PCR is used to make enough copies of the specific gene or fragment you want to clone.
Add Restriction Sites
Primers can be designed with extra sequences at their ends to add restriction enzyme sites. This makes it easier to insert the amplified DNA into a plasmid vector using traditional cloning.
Screen Clones
After cloning, PCR is often used to check whether a colony contains the right DNA insert — this is called colony PCR.
Mutagenesis and Gene Editing
PCR can also introduce small changes (mutations) to study how different DNA sequences affect gene function.
Advantages of PCR in Cloning
Fast: Millions of copies can be made in a few hours.
Sensitive: Can amplify DNA from a single cell or trace sample.
Versatile: Works for DNA fragments of many sizes and sources.
Precise: With well-designed primers, you can target exactly the DNA region you need
Modern PCR Innovations
Today, there are advanced PCR methods for molecular cloning:
High-Fidelity PCR: Uses DNA polymerases with proofreading ability for fewer errors — great for making accurate clones.
Overlap Extension PCR: Joins DNA fragments together without restriction enzymes.
qPCR (Real-Time PCR): Measures DNA amounts in real time but is more for quantification than cloning.
One-Step Cloning Kits: Combine PCR with ligation and transformation in a single tube.
Selectable Markers and Reporter Genes in Molecular Cloning
In molecular cloning, getting foreign DNA into a host cell is only half the battle you also need a way to find out which cells successfully took up your recombinant DNA. This is where selectable markers and reporter genes come in. They’re crucial tools that help scientists identify, select, and confirm the cells that carry the DNA of interest.
What Are Selectable Markers?
Selectable markers are genes added to the cloning vector (like a plasmid) that give host cells a survival advantage under specific conditions. The most common selectable markers provide antibiotic resistance, allowing only cells that contain the plasmid to survive when grown on antibiotic-containing media.
What Are Reporter Genes?
Reporter genes don’t provide survival advantages. Instead, they make it easy to see, measure, or detect whether your gene of interest is present and being expressed. Reporter genes produce a detectable product, like a color change, fluorescence, or enzymatic activity.
How Does m olecular Cloning Work?
Molecular cloning might sound complex, but the basic idea is simple. It has a few key steps:
1. Cutting the DNA
Scientists use special enzymes called restriction enzymes to cut the DNA at precise spots. This lets them isolate the exact gene or DNA piece they want.
2. Inserting the DNA into a Vector
The DNA piece is then inserted into a small circular DNA molecule called a plasmid vector. Plasmids can replicate independently inside bacteria, making them perfect carriers.
3. Joining the Pieces Together
An enzyme called DNA ligase acts like glue, sealing the DNA fragment into the plasmid to create recombinant DNA.
4. Putting it into Host Cells
The recombinant plasmid is introduced into host cells, usually bacteria like E. coli. These bacteria multiply rapidly, copying the plasmid and the inserted gene as they grow.
5. Selecting the Right Clones
Not every cell will take up the plasmid. So, scientists use selectable markers (like antibiotic resistance genes) to make sure only the bacteria with the new DNA survive.
6. Checking the Clones
To make sure everything worked, researchers test the colonies to see if they contain the right DNA. They may use reporter genes, sequencing, or other tools to verify the result.
Why Is Molecular Cloning Important?
Medicine
Making insulin, vaccines, and antibodies.
Agriculture
Engineering crops that resist pests or harsh conditions